Rapid Detection Test for Acute Respiratory Disease Marker Launched
Thermo Fisher Scientific’s company Oxoid introduced a new, simplified diagnostic test to screen for human metapneumovirus (hMPV), the Imagen hMPV, which is frequently implicated in acute respiratory tract disease. This test can be used in a basic laboratory setting as a routine screening test as detailed in the news release.
The test is completed with direct immunofluorescence laboratory analysis. Monoclonal antibodies are conjugated to a fluorescent dye, this detects the presence of viral antigens expressed by all strains of hMPV. The test only requires one step for analysis and uses common equipment found in the laboratory setting. With this simplified procedure, the test can be completed by most laboratory facilities.
Specimens are incubated for 15 minutes, then mounted on slides, and examined with epifluorescent illumination. Presence of infected cells is signified by a bright apple-green fluorescence during microscopic evaluation, presented against a red background stain of the cells without the virus.
Existing tests to detect hMPV generally employ either detection of viral RNA with polymerase chain reaction (PCR) or virus isolation in cell cultures. Both these analysis approaches require special equipment and are time consuming for laboratory staff. As a result, these tests are generally completed only in larger laboratory facilities.hMPV is a common cause of respiratory illness, ranging from mild upper respiratory diseases to severe lower respiratory disease; including pneumonia, bronchitis, and bronchiolitis.
October 25, 2007 Related topics: Laboratory, Pulmonary, Product Launches, Laboratory Equipment, Diagnostic